Gilson의 'Rapid purification of DNA Fragments using Gilson EXTRACTMAN® and Macherey Nagel NucleoMag® PCR'을 이용한 응용자료는 한국분석기기(주)에서 제공하였으며 주요 내용은 다음과 같다.
Introduction
Most workflows in molecular biology require a DNA clean up at some point. Whether it is a PCR or a linearized plasmid, clean up of DNA fragments is - in terms of numbers - among the top methods performed in molecular biology laboratories. In order to perform this task as time- and cost-efficiently as possible, MACHEREY‑NAGEL developed the NucleoMag® PCR kit, which uses superparamagnetic particles for DNA purification.
Here, we present a new approach for the clean up of DNA fragments from small volumes of liquid combining the innovative design of EXTRACTMAN®(Figure 1) with the convenience of a DNA purification using MACHEREY‑NAGEL’s proven NucleoMag® technology. Due to its small size, off-grid usability, and portability, EXTRACTMAN is ready whenever and wherever you need it.
Figure 1. EXTRACTMAN for processing of magnetic bead-based molecular biology kits.
Method
A 645 bp PCR fragment and a linearized pCDNA plasmid(5.4 kbp) were purified in four replicates per run using the NucleoMag® PCR kit on Gilson EXTRACTMAN. Results were compared to extractions performed in an automated manner on a KingFisher® Flex magnetic particle separator.
Product at a glance
Application Data
Purification of PCR fragments
EXTRACTMAN enables efficient purification of PCR fragments with the NucleoMag® PCR kit with a recovery rate comparable to an automated reference preparation.
Purification of linearized plasmids
EXTRACTMAN allows efficient purification of linearized plasmids from reaction mixtures with the NucleoMag® PCR kit. The final concentration is slightly higher than with an automated reference preparation due to a smaller elution volume.
The process of capture-slide-release-slide allows the user to quickly perform four isolations in parallel. During the bind step, DNA in the linearized plasmids or PCR fragments is selectively adsorbed to the PMPs.
PMP-bound DNA is then moved through wash buffer to the elution buffer using magnetic manipulation of the PMPs(Figure 2).
Unbound contaminants remain in the input well and nonspecific carryover is eliminated via washes. Each step in the process requires only seconds of handson time.
Figure 2. EXTRACTMAN DNA isolation process using ESP.
Trademarks
All product and company names are trademarks™ or registered® trademarks of their respective holders. Use of the trademark(s) in this document does not imply any affiliation with or endorsements by the trademark holder(s).
Notice
This application note has been produced and edited using information that was available at the time of publication.
This application note is subject to revision without prior notice.
Gilson의 'Rapid purification of DNA Fragments using Gilson EXTRACTMAN® and Macherey Nagel NucleoMag® PCR'에 대한 궁금한 내용은 본 원고자료를 제공한 한국분석기기(주)를 통하여 확인할 수 있다.
Reference(참고문헌): Gilson Application note 1032(pp1-2)
Model Name(모델명): EXTRACTMAN
The Person in Charge(담당자): Hyesook An
Maker(제조사): Gilson.
Country of Origin(원산지): USA
e-mail: kaisco1@kaisco.co.kr
Data Services(자료제공): KAISCO.
<이 기사는 사이언스21 매거진 2020년 12월호에 게재 되었습니다.>
Figure 1. EXTRACTMAN for processing of magnetic bead-based molecular biology kits.MethodA 645 bp PCR fragment and a linearized pCDNA plasmid(5.4 kbp) were purified in four replicates per run using the NucleoMag® PCR kit on Gilson EXTRACTMAN. Results were compared to extractions performed in an automated manner on a KingFisher® Flex magnetic particle separator.Product at a glance
Application Data
Purification of PCR fragmentsEXTRACTMAN enables efficient purification of PCR fragments with the NucleoMag® PCR kit with a recovery rate comparable to an automated reference preparation.
Purification of linearized plasmidsEXTRACTMAN allows efficient purification of linearized plasmids from reaction mixtures with the NucleoMag® PCR kit. The final concentration is slightly higher than with an automated reference preparation due to a smaller elution volume.The process of capture-slide-release-slide allows the user to quickly perform four isolations in parallel. During the bind step, DNA in the linearized plasmids or PCR fragments is selectively adsorbed to the PMPs.PMP-bound DNA is then moved through wash buffer to the elution buffer using magnetic manipulation of the PMPs(Figure 2).Unbound contaminants remain in the input well and nonspecific carryover is eliminated via washes. Each step in the process requires only seconds of handson time.
Figure 2. EXTRACTMAN DNA isolation process using ESP.TrademarksAll product and company names are trademarks™ or registered® trademarks of their respective holders. Use of the trademark(s) in this document does not imply any affiliation with or endorsements by the trademark holder(s).NoticeThis application note has been produced and edited using information that was available at the time of publication.This application note is subject to revision without prior notice.Gilson의 'Rapid purification of DNA Fragments using Gilson EXTRACTMAN® and Macherey Nagel NucleoMag® PCR'에 대한 궁금한 내용은 본 원고자료를 제공한 한국분석기기(주)를 통하여 확인할 수 있다.Reference(참고문헌): Gilson Application note 1032(pp1-2)Model Name(모델명): EXTRACTMANThe Person in Charge(담당자): Hyesook AnMaker(제조사): Gilson.Country of Origin(원산지): USAe-mail: kaisco1@kaisco.co.krData Services(자료제공): KAISCO.<이 기사는 사이언스21 매거진 2020년 12월호에 게재 되었습니다.>